| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Rhizobium etli | TGCATGTTCGGTTCGGCT | TTCAGAACGTCGTCCCAGC | 59.97 | 60.01 | 254 | 72 |
100.00%
|
25.00%
|
| Primer 22 | Rhizobium etli | TGGCGTCTGGAATACGAGC | AGAACGGCAGGTTGAGCA | 59.86 | 59.17 | 209 | 69 |
100.00%
|
25.00%
|
| Primer 23 | Rhizobium etli | TCGTGGAAAAGCACGGCA | CTTGGCTTTCAGCATCGCC | 60.20 | 59.86 | 251 | 69 |
100.00%
|
25.00%
|
| Primer 24 | Rhizobium etli | TCGTGGAAAAGCACGGCA | AGCCGTCGAGAATGAAGCC | 60.20 | 60.15 | 198 | 69 |
100.00%
|
25.00%
|
| Primer 25 | Rhizobium etli | AACTCAGCACGACGAGCA | GCGGATGCGGTTTTCGATC | 59.27 | 60.01 | 218 | 69 |
100.00%
|
25.00%
|
| Primer 26 | Rhizobium etli | AACTCAGCACGACGAGCA | GCGGATGCGGTTTTCGAT | 59.27 | 58.90 | 218 | 69 |
100.00%
|
25.00%
|
| Primer 27 | Rhizobium etli | TCTACGGCAATCGCATCGG | TCGGTGCGCATCAGGAAA | 60.30 | 59.65 | 226 | 68 |
100.00%
|
25.00%
|
| Primer 28 | Rhizobium etli | GCACCAACCTGCGCAATT | AGCCTTCGATGTCGCGTT | 59.66 | 59.74 | 222 | 68 |
100.00%
|
25.00%
|
| Primer 29 | Rhizobium etli | AGCTGACGCTTGCCGATT | AGCACGCTGAGCCGATATC | 60.05 | 60.01 | 203 | 68 |
100.00%
|
25.00%
|
| Primer 30 | Rhizobium etli | AGCTGACGCTTGCCGATT | GCTGACGATGGAGGCCATT | 60.05 | 60.15 | 260 | 68 |
100.00%
|
25.00%
|